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Stafford, Darrel W.

442 Wilson Hall
(919) 962-0597 (office)

My laboratory interests are in the broad area of molecular biology. Before the advent of molecular cloning we successfully isolated a pure gene from sea urchins (the ribosomal rRNA gene). We also purified to near homogeneity a gene for one of the sea urchin histones prior to cloning. A paper by Bob Simpson and myself was the first to demonstrate nucleosome phasing.

My major interest at present is the study of protein-protein interactions. At present, we are investigating interactions of the blood coagulation proteins in coagulation and in the control of the pathways of coagulation. Our approach is to use molecular modeling to suggest mutations and to do in vitro mutagenesis to express the proteins and characterize their interactions.

We recently purified the g-glutamyl carboxylase from bovine liver and have now cloned its cDNA. This opens a new avenue of research for us and others.