Below are recent publications by members of the Bloom Lab. Undergraduate researchers/authors are in bold.
He, Y.; Lawrimore, J.; Cook, D.; Van Gorder, E.E.; De Larminat, S.C.; Adalsteinsson, D.; Forest, MG; Bloom, K. (2020) Statistical mechanics of chromosomes: In vivo and in silico approaches reveal high-level organization and structure arise exclusively through mechanical feedback between loop extruders and chromatin substrate properties Nucleic Acids Res Nov 18;48(20):11284-11303 NAR-01822-N-2020.R2 doi: 10.1093/nar/gkaa871
- An in silico study that shows mechanical feedback between condensin and histones as they interact on chromatin with our collaborators in Applied Math and two of Biology’s undergraduates, Solenn De Larminat and Elizabeth Van Gorder.
Mishra, P., Chakraborty, A., Yeh, E., Feng, W., Bloom, K.S. and Basrai, M.A. (2021) R-loops at centromeric chromatin contribute to defects in kinetochore integrity and chromosomal instability in budding yeast Mol Biol Cell Jan 1;32(1):74-89. doi: 10.1091/mbc.E20-06-0379
- A study showing R-loops at the yeast centromere and its function in kinetochore integrity with our collaborators at NIH
Kefer, P., Iqbal, F., Locatelli, M., Lawrimore, J., Zhang, M. Bloom, K., Bonin, K., Liu, J., Vidi, P-A. (2021) Performance of deep learning restoration methods for the extraction of particle dynamics in noisy microscopy image sequences. Mol Biol Cell Jan 27:mbcE20110689. doi: 10.1091/mbc.E20-11-0689.
- A quantitative analysis tool using artificial intelligence methods with our collaborators at Wake Forest University
Cook, D., Long, S., Stanton, J., Cusick, P., Lawrimore, C., Yeh, E., Grant, S. and Bloom, K. (2021) Behavior of Dicentric Chromosomes in Budding Yeast. PLOS Genetics (accepted) PGENETICS-D-20-00674R2
- A study showing differential repair pathways for dicentric chromosomes depending on the position of the two centromeres. This study stemmed from a CURE course Bio 423 taught by Dr. Sarah Grant. The two undergraduates in the class, John Stanton and Patrick Cusick worked in Dr. Bloom’s lab as post-bacs to finish up the experiments.
Lawrimore, J., Kolbin, D., Stanton, J., Khan, Muznah, De Larminat, S., Lawrimore, C., Yeh, E., Bloom, K. (2021) The rDNA is Biomolecular Condensate Formed by Polymer-Polymer Phase Separation and is Sequestered in the Nucleolus by Transcription and R-loops. Nucl. Acids Res. (accepted).
- A study demonstrating the role of transcription and R-loops in preventing rDNA demixing from the liquid phase of the nucleolus. The study was initiated by a quantitative Biology undergraduate student, Muznah Khan, with assistance from two undergraduates, John Stanton and Solenn De Larminat.